Control of Rhizoctonia solani with extracts from Ovidia andina
New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen. Powdered dried leaves and stems were extracted by maceration with dichloromethane (CH2Cl2), methanol and ethanol. An aqueous...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
|---|---|
| Autores principales: | , , , , , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=12070 |
| todos_str_mv |
11479 78 eng Protección vegetal UBA UBA UBA UBA_FA UBA_FA UBA_FA UNSa |
|---|---|
| descriptores_str_mv |
Active fractions Bioguided fractionation CH2Cl2 extract Control de enfermedades de plantas Coumarins and flavonoids characterization Cumarinas Extracto CH2Cl2 Extractos Flavonoides Fracciones activas Fungal growth Hongos patógenos Hongos patógenos de suelo Ovidia andina Rhizoctonia solani |
| autor_str_mv |
Broussalis, Adriana M. Cufre, Ingrid M. Fabrizio, María C. Rivera, Marta C. Tarcaya, Verónica P. Tito Mansilla, Javier Wright, Eduardo R. |
| disciplina_str_mv |
Ciencias agrarias |
| titulo_str_mv |
Control de Rhizoctonia solani con extractos de Ovidia andina Control of Rhizoctonia solani with extracts from Ovidia andina |
| description_str_mv |
New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia
solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen.
Powdered dried leaves and stems were extracted by maceration with dichloromethane
(CH2Cl2), methanol and ethanol. An aqueous extract was obtained by decoction, and the
hydrodistillation residual water was the aqueous phase after essences production. A
strain of R. solani was cultivated on potato dextrose agar supplemented with the extracts,
at 1:100 and 1:1000 v/v. The CH2Cl2 extract at 1:100 reduced colony growth (p< 0.0001)
and changed hyphal morphology. Five fractions were obtained from the CH2Cl2 extract in
a glass column, and TLC analysis showed the presence of coumarins and flavonoids. The
CH2Cl2 extract and fractions were diluted in CH2Cl2 or acetone. The efficiency to reduce
pathogen growth of fractions 2f and 5f diluted in CH2Cl2 at 1:100 did not differ from the
whole extract (p<0.0001). For an in vivo test, beetroot seed balls were incubated in soil
infested with R. solani and treated by immersion in the CH2Cl2 extract at 1:100 for different
periods of time. As a result, pathogen colonization was diminished by immersion for 120 s
and seed germination was normal (p< 0.0001). Additionally, infested soil was treated with
the CH2Cl2 extract, and inoculum concentration estimated using beetroot seed balls as
baits diminished (p< 0.0001). These results lead us to conclude that O. andina is a source
of antifungal components for crop protection against R. solani. Resulta necesario generar alternativas para el control de hongos patógenos de suelo como Rhizoctonia solani. En este trabajo, se evaluó la actividad de extractos de Ovidia andina sobre una cepa del patógeno. Se realizaron extracciones a partir de hojas y tallos secos pulverizados, macerados con diclorometano (CH2Cl2), metanol y etanol. Se obtuvo un extracto acuoso por decocción y aguas residuales consistentes en la fase acuosa luego de la producción de esencias. Se cultivó una cepa de R. solani en agar papa glucosa suplementado con los extractos diluidos 1:100 o 1:1000 v/v. El extracto CH2Cl2 1:100 redujo el crecimiento de las colonias (p< 0,0001) y alteró la morfología de las hifas. Se obtuvieron 5 fracciones del extracto CH2Cl2 en una columna de vidrio y mediante TLC se detectó la presencia de cumarinas y flavonoides. Posteriormente, el extracto CH2Cl2 y sus fracciones se diluyeron en CH2Cl2 o acetona. La eficiencia de las fracciones 2f y 5f diluidas CH2Cl2 1:100 para reducir el crecimiento fúngico no difirió de la del extracto completo (p<0,0001). En un experimento in vivo, se incubaron glomérulos de remolacha en suelo infestado con R. solani y se sumergieron en el extracto CH2Cl2 1:100 durante tiempos variables. La colonización fúngica disminuyó mediante la inmersión durante 120 segundos, sin afectar la germinación (p< 0,0001). Adicionalmente, se trató suelo infestado con el extracto CH2Cl2 y disminuyó la concentración de inóculo del patógeno estimada utilizando glomérulos de remolacha como trampas (p< 0,0001). Estos resultados permiten concluir que O. andina es una fuente de componentes antifúngicos para la protección vegetal contra R. solani. |
| object_type_str_mv |
Textual: Revistas |
| id |
12070 |
| plantilla_str |
Artículo de Revista |
| record_format |
article |
| container_title |
Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
| journal_id_str |
r-78 |
| container_issue |
Revista de la Facultad de Ciencias Agrarias |
| container_volume |
Vol. 50, no. 2 |
| journal_issue_str |
Vol. 50, no. 2 |
| tipo_str |
textuales |
| type_str_mv |
Articulos |
| title_full |
Control of Rhizoctonia solani with extracts from Ovidia andina |
| title_fullStr |
Control of Rhizoctonia solani with extracts from Ovidia andina Control of Rhizoctonia solani with extracts from Ovidia andina |
| title_full_unstemmed |
Control of Rhizoctonia solani with extracts from Ovidia andina Control of Rhizoctonia solani with extracts from Ovidia andina |
| description |
New alternatives are needed to control ubiquitous soilborne fungi like Rhizoctonia
solani. This work evaluates the activity of extracts from Ovidia andina on this pathogen.
Powdered dried leaves and stems were extracted by maceration with dichloromethane
(CH2Cl2), methanol and ethanol. An aqueous extract was obtained by decoction, and the
hydrodistillation residual water was the aqueous phase after essences production. A
strain of R. solani was cultivated on potato dextrose agar supplemented with the extracts,
at 1:100 and 1:1000 v/v. The CH2Cl2 extract at 1:100 reduced colony growth (p< 0.0001)
and changed hyphal morphology. Five fractions were obtained from the CH2Cl2 extract in
a glass column, and TLC analysis showed the presence of coumarins and flavonoids. The
CH2Cl2 extract and fractions were diluted in CH2Cl2 or acetone. The efficiency to reduce
pathogen growth of fractions 2f and 5f diluted in CH2Cl2 at 1:100 did not differ from the
whole extract (p<0.0001). For an in vivo test, beetroot seed balls were incubated in soil
infested with R. solani and treated by immersion in the CH2Cl2 extract at 1:100 for different
periods of time. As a result, pathogen colonization was diminished by immersion for 120 s
and seed germination was normal (p< 0.0001). Additionally, infested soil was treated with
the CH2Cl2 extract, and inoculum concentration estimated using beetroot seed balls as
baits diminished (p< 0.0001). These results lead us to conclude that O. andina is a source
of antifungal components for crop protection against R. solani. |
| dependencia_str_mv |
Facultad de Ciencias Agrarias |
| title |
Control of Rhizoctonia solani with extracts from Ovidia andina |
| spellingShingle |
Control of Rhizoctonia solani with extracts from Ovidia andina Active fractions Bioguided fractionation CH2Cl2 extract Control de enfermedades de plantas Coumarins and flavonoids characterization Cumarinas Extracto CH2Cl2 Extractos Flavonoides Fracciones activas Fungal growth Hongos patógenos Hongos patógenos de suelo Ovidia andina Rhizoctonia solani Broussalis, Adriana M. Cufre, Ingrid M. Fabrizio, María C. Rivera, Marta C. Tarcaya, Verónica P. Tito Mansilla, Javier Wright, Eduardo R. |
| topic |
Active fractions Bioguided fractionation CH2Cl2 extract Control de enfermedades de plantas Coumarins and flavonoids characterization Cumarinas Extracto CH2Cl2 Extractos Flavonoides Fracciones activas Fungal growth Hongos patógenos Hongos patógenos de suelo Ovidia andina Rhizoctonia solani |
| topic_facet |
Active fractions Bioguided fractionation CH2Cl2 extract Control de enfermedades de plantas Coumarins and flavonoids characterization Cumarinas Extracto CH2Cl2 Extractos Flavonoides Fracciones activas Fungal growth Hongos patógenos Hongos patógenos de suelo Ovidia andina Rhizoctonia solani |
| author |
Broussalis, Adriana M. Cufre, Ingrid M. Fabrizio, María C. Rivera, Marta C. Tarcaya, Verónica P. Tito Mansilla, Javier Wright, Eduardo R. |
| author_facet |
Broussalis, Adriana M. Cufre, Ingrid M. Fabrizio, María C. Rivera, Marta C. Tarcaya, Verónica P. Tito Mansilla, Javier Wright, Eduardo R. |
| title_sort |
Control of Rhizoctonia solani with extracts from Ovidia andina |
| title_short |
Control of Rhizoctonia solani with extracts from Ovidia andina |
| url |
https://bdigital.uncu.edu.ar/fichas.php?idobjeto=12070 |
| estado_str |
3 |
| building |
Biblioteca Digital |
| filtrotop_str |
Biblioteca Digital |
| collection |
Artículo de Revista |
| institution |
Sistema Integrado de Documentación |
| indexed_str |
2023-04-25 00:35 |
| _version_ |
1764120174896611328 |
