Papel del entorno celular sobre la progresión del cáncer renal
Between the epithelial tissue and the fibroblastic / adipose stroma an essential exchange of information is established for normal morphogenesis and functionality, as well as for the development of cancer. We study kidney cancer because it is the fifth most common type of cancer worldwide. It is als...
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| Autores principales: | , , , , , , |
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| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=14145 |
| Sumario: | Between the epithelial tissue and the fibroblastic / adipose stroma an essential exchange of information is established for normal morphogenesis and functionality, as well as for the development of cancer. We study kidney cancer because it is the fifth most common type of cancer worldwide. It is also associated with high mortality and its incidence has increased significantly in recent years. We recently demonstrated that human adipose tissue from renal cell carcinoma near the tumor, regulates the behavior of tumor and non-tumor human renal epithelial cells differently than adipose tissue farther away from the tumor (Campo-Verde-Arbocco et al., 2017). Then, we started to work with fragments of human renal adipose tissue from patients with renal tumors (ARTh), and normal renal adipose tissue, from living donors of the kidney (ARNh). We found that ARTh expresses significantly higher amounts of leptin, ObR and versican, as well as significantly lower amounts of perilipin, adiponectin and AdipoR1, relative to ARNh. In addition, conditioned media (MCs) of ARTh increased the metastatic capacity of tumor and non-tumor renal human epithelial cells. In this project we intend to continue with the study of the ARNh and ARTh fragments; as well as the effects of both types of tissue on the development of the renal tumor. Specifically, we will study the ability of different MCs to stimulate the angiogenesis process and evaluate the presence of exosomes in the different MCs. In addition, we will study the expression of different browning markers in ARTh and ARNh. On the other hand, we will evaluate changes in the expression of different cellular markers of epithelial-mesenchymal transition of epithelial cells when incubated with MCs-ARTh and -ARNh; and changes in morphogenesis and alterations in the migration / invasion of the renal epithelial cells co-cultured with fragments of the different adipose tissues. Finally, we will obtain the production of primary cultures of human tumor renal epithelial cells, from fragments of renal tumors obtained by surgical intervention. |
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