Generación de nanoanticuerpos empleando la tecnología de "phage display" para la detección de Chlamydia Trachomatis
Infections caused by the sexually-transmitted bacterium Chlamydia trachomatis are a major public health concern for its high incidence and prevalence among the population. The lack of symptoms makes clinical diagnosis difficult while the laboratory methods are complex (PCR, cell culture) or poorly s...
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| Autores principales: | , , , , |
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| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=14774 |
| Sumario: | Infections caused by the sexually-transmitted bacterium Chlamydia trachomatis are a major public health concern for its high incidence and prevalence among the population. The lack of symptoms makes clinical diagnosis difficult while the laboratory methods are complex (PCR, cell culture) or poorly sensitive (IF, ELISA). A large proportion of infections are persistent and generate chronic inflammation, with irreversible sequelae such as scarring and obstruction of the fallopian tubes, leading to ectopic pregnancies or female infertility. We propose to introduce here the technology of production of nanoantibodies by phage display. These small recombinant antibodies have the advantage of being produced completely in vitro, in bacteria, without the need for mice or rabbits, reducing costs and time of production. In 2018, Smith and Winter were awarded the Nobel Prize in Chemistry for the discovery of nanoantibody production technology, whereas Honjo and Allison earned the Nobel Prize in Medicine for its application in immunotherapy. The specificity and resistance to the degradation of the nanoantibodies favor its use for the diagnosis of some diseases and the presence of infectious agents. To use screening tests, highly sensitive and specific, easy to perform and cheap, is fundamental for succeeding in the detection of sexually-transmitted diseases. Therefore, we consider that the generation of nanoantibodies would be useful for the development of screening tests for the diagnosis of chlamydial infections. It would also be a very advantageous tool for the laboratory since we specialize in the study of the molecular mechanisms that determine the establishment of chlamydial infections, and how the bacteria adapt and parasitize the cells that they infect. |
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