Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes:
Gamma irradiation has been widely used as a breeding technique to obtain new cultivars in ornamental species such as Alstroemeria, where several cultivars have been obtained through rhizome radiation. The optimum dosage for an appropriate induction of mutation must be considered for breeding p...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
|---|---|
| Autores principales: | , , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=4523 |
| descriptores_str_mv |
Breeding Brotación Chile Irradiación gamma Mejoramiento genético Mutación Mutation Radio susceptibilidad Radio susceptibility Sprouting |
|---|---|
| todos_str_mv |
4482 78 eng Pontificia Universidad Católica, Chile UCh_FCA UCh_FCA UCh_FCA |
| autor_str_mv |
Aros, Danilo Infante, Rodrigo Olate, Eduardo Valdés, Santiago |
| disciplina_str_mv |
Ciencias agrarias |
| description_str_mv |
Gamma irradiation has been widely used
as a breeding technique to obtain new cultivars
in ornamental species such as Alstroemeria,
where several cultivars have been obtained
through rhizome radiation. The optimum dosage
for an appropriate induction of mutation must be
considered for breeding purposes and it depends
mainly on plant susceptibility. Thus in this study
in vitro cultured rhizomes of Alstroemeria aurea
were irradiated with a gamma source using
different dosages to evaluate the direct effect
produced. Damage and number of rhizome
sprouting were observed and recorded during
61 days after irradiation. At the end of this period,
rhizomes were weighted and mortality was
evaluated. Both mortality and weight increased
depending on dosage. All irradiated rhizomes
showed early sprouting in comparison with
control (0 Gy) and no significant difference in final
number of shoots after 61 days among irradiated
treatments was observed. Bleaching and necrosis
was observed in all irradiated rhizomes and
was more evident at higher doses. LD50 was
established at about 40 Gy and the optimum
dosage to induce mutation was suggested
between 2.5 and 5 Gy, when the growth was
reduced in 50%, and probably this dosage could
be used for breeding purposes.
La irradiación con rayos gamma ha sido ampliamente utilizada como herramienta en mejoramiento genético para obtener nuevos cultivares de especies ornamentales tales como Alstroemeria, en que varios cultivares se han obtenido mediante la irradiación de rizomas. La dosis óptima para una apropiada inducción de mutación debe ser considerada para fines de mejoramiento genético y depende principalmente de la susceptibilidad de la planta. En este estudio, rizomas de Alstroemeria aurea cultivados in vitro fueron irradiados con una fuente de rayos gamma utilizando diferentes dosis para evaluar el efecto directo producido. El daño y número de rizomas en brotación fueron observados y registrados durante 61 días después de la irradiación. Al final de este periodo, los rizomas fueron pesados y la mortalidad fue evaluada. Tanto la mortalidad como el peso aumentaron dependiendo de la dosis. Todos los rizomas irradiados mostraron una brotación anticipada en comparación con el control (0 Gy) y no se observaron diferencias significativas en el número final de brotes después de los 61 días entre los diferentes tratamientos. Se observó blanqueamiento y necrosis en todos los rizomas irradiados, siendo más evidente a altas dosis. El DL50 fue establecido alrededor de 40 Gy y la dosis óptima para inducir mutación se sugiere entre 2,5 y 5 Gy, cuando el crecimiento se redujo en un 50%, y probablemente esta dosis pueda ser utilizada con fines de mejoramiento genético. |
| titulo_str_mv |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: Irradiación de rizomas in vitro de Alstroemeria aurea G. con rayos gamma: |
| object_type_str_mv |
Textual: Revistas |
| id |
4523 |
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Artículo de Revista |
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article |
| container_title |
Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
| journal_id_str |
r-78 |
| container_issue |
Revista de la Facultad de Ciencias Agrarias |
| container_volume |
Vol. 44, no. 1 |
| journal_issue_str |
Vol. 44, no. 1 |
| tipo_str |
textuales |
| type_str_mv |
Articulos |
| title_full |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: |
| title_fullStr |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: |
| title_full_unstemmed |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: |
| description |
Gamma irradiation has been widely used
as a breeding technique to obtain new cultivars
in ornamental species such as Alstroemeria,
where several cultivars have been obtained
through rhizome radiation. The optimum dosage
for an appropriate induction of mutation must be
considered for breeding purposes and it depends
mainly on plant susceptibility. Thus in this study
in vitro cultured rhizomes of Alstroemeria aurea
were irradiated with a gamma source using
different dosages to evaluate the direct effect
produced. Damage and number of rhizome
sprouting were observed and recorded during
61 days after irradiation. At the end of this period,
rhizomes were weighted and mortality was
evaluated. Both mortality and weight increased
depending on dosage. All irradiated rhizomes
showed early sprouting in comparison with
control (0 Gy) and no significant difference in final
number of shoots after 61 days among irradiated
treatments was observed. Bleaching and necrosis
was observed in all irradiated rhizomes and
was more evident at higher doses. LD50 was
established at about 40 Gy and the optimum
dosage to induce mutation was suggested
between 2.5 and 5 Gy, when the growth was
reduced in 50%, and probably this dosage could
be used for breeding purposes.
|
| dependencia_str_mv |
Facultad de Ciencias Agrarias |
| title |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: |
| spellingShingle |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: Breeding Brotación Chile Irradiación gamma Mejoramiento genético Mutación Mutation Radio susceptibilidad Radio susceptibility Sprouting Aros, Danilo Infante, Rodrigo Olate, Eduardo Valdés, Santiago |
| topic |
Breeding Brotación Chile Irradiación gamma Mejoramiento genético Mutación Mutation Radio susceptibilidad Radio susceptibility Sprouting |
| topic_facet |
Breeding Brotación Chile Irradiación gamma Mejoramiento genético Mutación Mutation Radio susceptibilidad Radio susceptibility Sprouting |
| author |
Aros, Danilo Infante, Rodrigo Olate, Eduardo Valdés, Santiago |
| author_facet |
Aros, Danilo Infante, Rodrigo Olate, Eduardo Valdés, Santiago |
| title_sort |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: |
| title_short |
Gamma irradiation on Alstroemeria aurea G. in vitro rhizomes: |
| url |
https://bdigital.uncu.edu.ar/fichas.php?idobjeto=4523 |
| estado_str |
3 |
| building |
Biblioteca Digital |
| filtrotop_str |
Biblioteca Digital |
| collection |
Artículo de Revista |
| institution |
Sistema Integrado de Documentación |
| indexed_str |
2023-04-25 00:38 |
| _version_ |
1764120313354780672 |
