Conidial germination of Botryosphaeria dothidea Mough.: Fr (Ces. & De Not.) and histological alterations on stems of pitahaya (Hylocereus undatus H.) (Haworth) Britton & Rose
Conidial germination of Botryosphaeria dothidea (anamorph: Fusicoccum) in sterile distilled water and 1% sterile dextrose solution was evaluated at 4, 6, 12, 24 and 36 h after incubation. Also, it was described the anatomical changes on pitahaya stems induced by this fungus, collected in the f...
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| Publicado en: | Revista de la Facultad de Ciencias Agrarias |
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| Autores principales: | , , |
| Materias: | |
| Acceso en línea: | https://bdigital.uncu.edu.ar/fichas.php?idobjeto=5037 |
| Sumario: | Conidial germination of Botryosphaeria
dothidea (anamorph: Fusicoccum) in sterile
distilled water and 1% sterile dextrose solution
was evaluated at 4, 6, 12, 24 and 36 h after
incubation. Also, it was described the anatomical
changes on pitahaya stems induced by this
fungus, collected in the field and artificially
inoculated in the laboratory. Conidial germination
was less than 30% in water and it was improved
when 1% dextrose was added to the water.
In 1% dextrose solution the germination was
90% after 4h of incubation and 100% at 6 h.
Pathogen germ tubes had entered through
wounds and sometimes through stomata and
hyphae colonized intra and intercellularly in
the parenchyma-chlorenchyma tissues. On
naturally and artificially diseased stems the
main alterations were: destruction of cuticle,
hyperplasia of epidermal and collenchymatous
hypodermal cells and conform the advance
of the pathogen a layer of lignified periderm
was formed surrounding the damaged tissues;
however, it couldn't stop the advance of the
pathogen and the cells that surrounded the
lesion suffered necrosis. |
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