Análisis mediante FTIR de la conservación del colágeno y la posible contaminación en muestras óseas del Cuaternario pampeano

This paper analyzes FTIR (infrared spectroscopy by Fourier transform) spectra of type I collagen samples extracted from modern and archaeological bones of guanaco (Lama guanicoe) and Pampas deer (Ozotoceros bezoarticus) with varying degrees of protein conservation and, in some cases, external organi...

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Detalles Bibliográficos
Autores principales: Vitale, Paula, Tasca, Julia, Bax, Marcela, Ariadna Flores, Politis, Gustavo G., Valenzuela, Luciano
Formato: Online
Lenguaje:spa
Publicado: Facultad de Filosofía y Letras, Universidad Nacional de Cuyo 2019
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Acceso en línea:https://revistas.uncu.edu.ar/ojs3/index.php/analarqueyetno/article/view/3737
Descripción
Sumario:This paper analyzes FTIR (infrared spectroscopy by Fourier transform) spectra of type I collagen samples extracted from modern and archaeological bones of guanaco (Lama guanicoe) and Pampas deer (Ozotoceros bezoarticus) with varying degrees of protein conservation and, in some cases, external organic input. C:N atomic ratio, C%, N%, and collagen yield were determined. Four transmittance ratios from the spectra were analyzed: Amide III/Amide I, Amide III/Proline, Proline/Amide I, 1080 cm-1/Amide I, which we propose be used for determining collagen quality. Since FTIR analysis is very versatile, it can be used as a simple decision-making tool, it is inexpensive, rapid, and available, we argue that establishing quality parameters with this method would allow us to determine collagen preservation in archaeological samples before sending them for isotopic and radiometric analyses and select the best samples.