Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L.
This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion...
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Facultad de Ciencias Agrarias-UNCuyo
2019
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2337 |
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I11-R107article-23372019-12-18T16:13:54Z Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge reguladores del crecimiento germinación organogénesis callogénesis plant growth germination organogenesis callogenesis This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion times for seed disinfection; for seed germination in vitro GA3 was added to the MS basal culture medium in different concentrations, and for morphogenic processes in vitro, different concentrations of auxins and cytokinins were combined. The decrease in the contamination rate was with high concentrations of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1 BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0 mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were generated in Cinchona officinalis L, for the preservation and conservation of the species. This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion times for seed disinfection; for seed germination in vitro GA3 was added to the MS basal culture medium in different concentrations, and for morphogenic processes in vitro, different concentrations of auxins and cytokinins were combined. The decrease in the contamination rate was with high concentrations of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1 BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0 mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were generated in Cinchona officinalis L, for the preservation and conservation of the species. Facultad de Ciencias Agrarias-UNCuyo 2019-06-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2337 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 51 No. 1 (2019): January-June; 55-68 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 51 Núm. 1 (2019): Enero-Junio; 55-68 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2337/1711 https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2337/1712 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
| language |
eng |
| format |
Online |
| author |
Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge |
| spellingShingle |
Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. reguladores del crecimiento germinación organogénesis callogénesis plant growth germination organogenesis callogenesis |
| author_facet |
Moreno Serrano, José Antonio Pérez Ruíz, César Moreno Fierro, Ivonne Moreno Fierro, Jorge |
| author_sort |
Moreno Serrano, José Antonio |
| title |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| title_short |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| title_full |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| title_fullStr |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| title_full_unstemmed |
Effect of culture medium on morphogenic processes in vitro in Cinchona officinalis L. |
| title_sort |
effect of culture medium on morphogenic processes in vitro in cinchona officinalis l. |
| description |
This paper describes the effect of the various plant growth regulators of the culture medium on morphogenic processes in vitro in Cinchona officinalis L, a highly vulnerable species from southern Ecuador. To do this, different concentrations of NaOCl were used in combination with different immersion times for seed disinfection; for seed germination in vitro GA3 was added to the MS basal culture medium in different concentrations, and for morphogenic processes in vitro, different concentrations of auxins and cytokinins were combined. The decrease in the contamination rate was with high concentrations of NaOCl and an increase in the germination rate in 45 days with the addition of 1.0 mg L-1 GA3 to the culture medium the hormonal combination of 0.5 mg L-1 NAA + 2.5 mg L-1 BAP showed a high rate of shoot proliferation and with 1.0 mg L-1 NAA a high number of roots was obtained. In the callogenesis phase, the best results were obtained with 1.0 mg L-1 2,4-D + 0.5 mg L-1 BAP for callus proliferation. In vitro propagation protocols were generated in Cinchona officinalis L, for the preservation and conservation of the species. |
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2019 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/2337 |
| topic |
reguladores del crecimiento germinación organogénesis callogénesis plant growth germination organogenesis callogenesis |
| topic_facet |
reguladores del crecimiento germinación organogénesis callogénesis plant growth germination organogenesis callogenesis |
| work_keys_str_mv |
AT morenoserranojoseantonio effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT perezruizcesar effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT morenofierroivonne effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl AT morenofierrojorge effectofculturemediumonmorphogenicprocessesinvitroincinchonaofficinalisl |
| _version_ |
1800220875094491136 |
