Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico

The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations by...

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Autores principales: López-Lima, Daniel, Carrión, Gloria, Sánchez-Nava, Petra, Desgarennes, Damaris, Villain, Luc
Formato: Online
Lenguaje:eng
Publicado: Facultad de Ciencias Agrarias-UNCuyo 2020
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Acceso en línea:https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3075
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author López-Lima, Daniel
Carrión, Gloria
Sánchez-Nava, Petra
Desgarennes, Damaris
Villain, Luc
spellingShingle López-Lima, Daniel
Carrión, Gloria
Sánchez-Nava, Petra
Desgarennes, Damaris
Villain, Luc
Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
Coffea arabica
nematodo agallador
hongos filamentosos
Coffea arabica
root-knot nematodes
filamentous fungi
author_facet López-Lima, Daniel
Carrión, Gloria
Sánchez-Nava, Petra
Desgarennes, Damaris
Villain, Luc
author_sort López-Lima, Daniel
title Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
title_short Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
title_full Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
title_fullStr Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
title_full_unstemmed Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico
title_sort fungal diversity and fusarium oxysporum pathogenicity associated with coffee corky-root disease in mexico
description The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations by corky-root disease. Previous studies have revealed the presence of fungi in coffee corky-root tissues. However, these fungi have not been yet identified. This work aimed to identify at species level the fungi associated to the coffee corky-root symptoms and determine their pathogenicity on coffee plants. Coffee roots with corky-root symptoms were collected in eight sites distributed through the major coffee growing region of Veracruz. Observations of inside cortical root tissues under scanning electron microscope revealed abundant mycelium and conidia incorky-root samples in contrast with absence of any fungi development in healthy roots. Forty-nine fungi strains from internal corky-root tissue were isolated and identified at species level by ITS sequences. Fusarium oxysporum was the most frequent species and the only present in all of the corky-root samples. These strains were selected for the pathogenicity test. All F. oxysporum strains colonized the vascular system of coffee plants although none caused wilting symptoms. Highlights Fusarium oxysporum is a major fungus associated with coffee corky-root disease. None of the oxysporum strains inoculated into the roots of coffee plants caused vascular withering symptoms In the tissues damaged by coffee corky-root disease interact nematodes, fungi and bacteria.
publisher Facultad de Ciencias Agrarias-UNCuyo
publishDate 2020
url https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3075
topic Coffea arabica
nematodo agallador
hongos filamentosos
Coffea arabica
root-knot nematodes
filamentous fungi
topic_facet Coffea arabica
nematodo agallador
hongos filamentosos
Coffea arabica
root-knot nematodes
filamentous fungi
work_keys_str_mv AT lopezlimadaniel fungaldiversityandfusariumoxysporumpathogenicityassociatedwithcoffeecorkyrootdiseaseinmexico
AT carriongloria fungaldiversityandfusariumoxysporumpathogenicityassociatedwithcoffeecorkyrootdiseaseinmexico
AT sancheznavapetra fungaldiversityandfusariumoxysporumpathogenicityassociatedwithcoffeecorkyrootdiseaseinmexico
AT desgarennesdamaris fungaldiversityandfusariumoxysporumpathogenicityassociatedwithcoffeecorkyrootdiseaseinmexico
AT villainluc fungaldiversityandfusariumoxysporumpathogenicityassociatedwithcoffeecorkyrootdiseaseinmexico
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spelling I11-R107article-30752020-07-06T14:59:47Z Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico Fungal diversity and Fusarium oxysporum pathogenicity associated with coffee corky-root disease in Mexico López-Lima, Daniel Carrión, Gloria Sánchez-Nava, Petra Desgarennes, Damaris Villain, Luc Coffea arabica nematodo agallador hongos filamentosos Coffea arabica root-knot nematodes filamentous fungi The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations by corky-root disease. Previous studies have revealed the presence of fungi in coffee corky-root tissues. However, these fungi have not been yet identified. This work aimed to identify at species level the fungi associated to the coffee corky-root symptoms and determine their pathogenicity on coffee plants. Coffee roots with corky-root symptoms were collected in eight sites distributed through the major coffee growing region of Veracruz. Observations of inside cortical root tissues under scanning electron microscope revealed abundant mycelium and conidia incorky-root samples in contrast with absence of any fungi development in healthy roots. Forty-nine fungi strains from internal corky-root tissue were isolated and identified at species level by ITS sequences. Fusarium oxysporum was the most frequent species and the only present in all of the corky-root samples. These strains were selected for the pathogenicity test. All F. oxysporum strains colonized the vascular system of coffee plants although none caused wilting symptoms. Highlights Fusarium oxysporum is a major fungus associated with coffee corky-root disease. None of the oxysporum strains inoculated into the roots of coffee plants caused vascular withering symptoms In the tissues damaged by coffee corky-root disease interact nematodes, fungi and bacteria. The disease known as coffee corky-roots associated to the infection by the root-knot nematode Meloidogyne paranaensis is an important issue for coffee crop in several countries. In Mexico, particularly in the Veracruz state, considerable loses are recorded annually in Coffea arabica plantations by corky-root disease. Previous studies have revealed the presence of fungi in coffee corky-root tissues. However, these fungi have not been yet identified. This work aimed to identify at species level the fungi associated to the coffee corky-root symptoms and determine their pathogenicity on coffee plants. Coffee roots with corky-root symptoms were collected in eight sites distributed through the major coffee growing region of Veracruz. Observations of inside cortical root tissues under scanning electron microscope revealed abundant mycelium and conidia incorky-root samples in contrast with absence of any fungi development in healthy roots. Forty-nine fungi strains from internal corky-root tissue were isolated and identified at species level by ITS sequences. Fusarium oxysporum was the most frequent species and the only present in all of the corky-root samples. These strains were selected for the pathogenicity test. All F. oxysporum strains colonized the vascular system of coffee plants although none caused wilting symptoms. Highlights Fusarium oxysporum is a major fungus associated with coffee corky-root disease. None of the oxysporum strains inoculated into the roots of coffee plants caused vascular withering symptoms In the tissues damaged by coffee corky-root disease interact nematodes, fungi and bacteria. Facultad de Ciencias Agrarias-UNCuyo 2020-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3075 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 No. 1 (2020): January-June; 276-292 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 Núm. 1 (2020): Enero-Junio; 276-292 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3075/2225 https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3075/2625