Quantification of isoflavones in stems of faba bean (Vicia faba L.)
The isoflavone aglycones daidzein and genistein are phytoestrogens that ameliorate the symptoms of menopause and assist in the prevention of chronic diseases. These compounds have been studied mainly in soya but have also been detected in faba beans (Vicia faba L.). Since little information is avai...
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| Formato: | Online |
| Lenguaje: | eng |
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Facultad de Ciencias Agrarias-UNCuyo
2020
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3791 |
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I11-R107article-37912020-12-04T21:59:31Z Quantification of isoflavones in stems of faba bean (Vicia faba L.) Quantification of isoflavones in stems of faba bean (Vicia faba L.) Fuentes-Herrera, Paula Beatriz Delgado-Alvarado, Adriana Herrera-Cabrera, Braulio Edgar Luna-Guevara, María Lorena Olvera-Hernández, José Isabel daidzeína genisteína cromatografía en capa fina cromatografía líquida de alta resolución daidzein genistein thin layer chromatography high-performance liquid chromatography The isoflavone aglycones daidzein and genistein are phytoestrogens that ameliorate the symptoms of menopause and assist in the prevention of chronic diseases. These compounds have been studied mainly in soya but have also been detected in faba beans (Vicia faba L.). Since little information is available concerning the content of isoflavone aglycones in faba beans from Mexico, the present study aimed to establish the presence and concentrations of daidzein and genistein in stems of faba bean cultivars from the Mexican central plateau. Cultivars C-281, C-146, C-160, C-89, C-288, C-181, C-93 and C-Zac22 were grown under greenhouse conditions and 6 to 7 week-old stems were harvested. The presence of daidzein and genistein in these tissues was confirmed by thin layer chromatography using a solvent system containing toluene-ethyl acetate-acetone-formic acid (74:14.8:7.4:3.8). High-pressure liquid chromatographic analysis revealed significant differences (p≤0.01) in the concentrations of aglycones with levels of genistein in the range 0.30 to 0.85 mg kg-1 dry weight (DW) and of daidzein, which was more abundant in all cultivars, present in the concentration range 34.92 to 59.98 mg kg-1 DW. Stems of cultivars C-89, C-181, C-281 and C-146 were exceptional for their concentrations of isoflavones and may represent good alternative sources of phytoestrogens. The isoflavone aglycones daidzein and genistein are phytoestrogens that ameliorate the symptoms of menopause and assist in the prevention of chronic diseases. These compounds have been studied mainly in soya but have also been detected in faba beans (Vicia faba L.). Since little information is available concerning the content of isoflavone aglycones in faba beans from Mexico, the present study aimed to establish the presence and concentrations of daidzein and genistein in stems of faba bean cultivars from the Mexican central plateau. Cultivars C-281, C-146, C-160, C-89, C-288, C-181, C-93 and C-Zac22 were grown under greenhouse conditions and 6 to 7 week-old stems were harvested. The presence of daidzein and genistein in these tissues was confirmed by thin layer chromatography using a solvent system containing toluene-ethyl acetate-acetone-formic acid (74:14.8:7.4:3.8). High-pressure liquid chromatographic analysis revealed significant differences (p≤0.01) in the concentrations of aglycones with levels of genistein in the range 0.30 to 0.85 mg kg-1 dry weight (DW) and of daidzein, which was more abundant in all cultivars, present in the concentration range 34.92 to 59.98 mg kg-1 DW. Stems of cultivars C-89, C-181, C-281 and C-146 were exceptional for their concentrations of isoflavones and may represent good alternative sources of phytoestrogens. Facultad de Ciencias Agrarias-UNCuyo 2020-12-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3791 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 No. 2 (2020): June-December; 43-51 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 Núm. 2 (2020): Julio-Diciembre; 43-51 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3791/2710 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
| language |
eng |
| format |
Online |
| author |
Fuentes-Herrera, Paula Beatriz Delgado-Alvarado, Adriana Herrera-Cabrera, Braulio Edgar Luna-Guevara, María Lorena Olvera-Hernández, José Isabel |
| spellingShingle |
Fuentes-Herrera, Paula Beatriz Delgado-Alvarado, Adriana Herrera-Cabrera, Braulio Edgar Luna-Guevara, María Lorena Olvera-Hernández, José Isabel Quantification of isoflavones in stems of faba bean (Vicia faba L.) daidzeína genisteína cromatografía en capa fina cromatografía líquida de alta resolución daidzein genistein thin layer chromatography high-performance liquid chromatography |
| author_facet |
Fuentes-Herrera, Paula Beatriz Delgado-Alvarado, Adriana Herrera-Cabrera, Braulio Edgar Luna-Guevara, María Lorena Olvera-Hernández, José Isabel |
| author_sort |
Fuentes-Herrera, Paula Beatriz |
| title |
Quantification of isoflavones in stems of faba bean (Vicia faba L.) |
| title_short |
Quantification of isoflavones in stems of faba bean (Vicia faba L.) |
| title_full |
Quantification of isoflavones in stems of faba bean (Vicia faba L.) |
| title_fullStr |
Quantification of isoflavones in stems of faba bean (Vicia faba L.) |
| title_full_unstemmed |
Quantification of isoflavones in stems of faba bean (Vicia faba L.) |
| title_sort |
quantification of isoflavones in stems of faba bean (viciaâ faba l.) |
| description |
The isoflavone aglycones daidzein and genistein are phytoestrogens that ameliorate the symptoms of menopause and assist in the prevention of chronic diseases. These compounds have been studied mainly in soya but have also been detected in faba beans (Vicia faba L.). Since little information is available concerning the content of isoflavone aglycones in faba beans from Mexico, the present study aimed to establish the presence and concentrations of daidzein and genistein in stems of faba bean cultivars from the Mexican central plateau. Cultivars C-281, C-146, C-160, C-89, C-288, C-181, C-93 and C-Zac22 were grown under greenhouse conditions and 6 to 7 week-old stems were harvested. The presence of daidzein and genistein in these tissues was confirmed by thin layer chromatography using a solvent system containing toluene-ethyl acetate-acetone-formic acid (74:14.8:7.4:3.8). High-pressure liquid chromatographic analysis revealed significant differences (p≤0.01) in the concentrations of aglycones with levels of genistein in the range 0.30 to 0.85 mg kg-1 dry weight (DW) and of daidzein, which was more abundant in all cultivars, present in the concentration range 34.92 to 59.98 mg kg-1 DW. Stems of cultivars C-89, C-181, C-281 and C-146 were exceptional for their concentrations of isoflavones and may represent good alternative sources of phytoestrogens. |
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2020 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3791 |
| topic |
daidzeína genisteína cromatografía en capa fina cromatografía líquida de alta resolución daidzein genistein thin layer chromatography high-performance liquid chromatography |
| topic_facet |
daidzeína genisteína cromatografía en capa fina cromatografía líquida de alta resolución daidzein genistein thin layer chromatography high-performance liquid chromatography |
| work_keys_str_mv |
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| _version_ |
1800220923101446144 |
