First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina
Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet po...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online |
| Lenguaje: | eng |
| Publicado: |
Facultad de Ciencias Agrarias-UNCuyo
2021
|
| Materias: | |
| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/4898 |
| id |
I11-R107article-4898 |
|---|---|
| record_format |
ojs |
| institution |
Universidad Nacional de Cuyo |
| building |
Revistas en línea |
| filtrotop_str |
Revistas en línea |
| collection |
Revista de la Facultad de Ciencias Agrarias |
| journal_title_str |
Revista de la Facultad de Ciencias Agrarias |
| institution_str |
I-11 |
| repository_str |
R-107 |
| language |
eng |
| format |
Online |
| author |
Martino, Julia A. Di Feo, Liliana del Valle Paccioretti, Mauro Contardi, Clara Adriana Sanchez, Miguel A. Ortega, Leandro I. Pastor, Silvina E. |
| spellingShingle |
Martino, Julia A. Di Feo, Liliana del Valle Paccioretti, Mauro Contardi, Clara Adriana Sanchez, Miguel A. Ortega, Leandro I. Pastor, Silvina E. First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina Ipomoea batatas podredumbre de raíz Chalara elegans enfermedad fúngica en batata Ipomoea batatas root rot Chalara elegans potato sweet potato fungal disease |
| author_facet |
Martino, Julia A. Di Feo, Liliana del Valle Paccioretti, Mauro Contardi, Clara Adriana Sanchez, Miguel A. Ortega, Leandro I. Pastor, Silvina E. |
| author_sort |
Martino, Julia A. |
| title |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_short |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_full |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_fullStr |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_full_unstemmed |
First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina |
| title_sort |
first report of berkeleyomyces basicola (synonymous: thielaviopsis basicola) on roots of sweet potato (ipomoea batatas (l.) lam) in argentina |
| description |
Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields.
Highlights:
Sweet potato black root rot, new disease in Argentina.
First report of Berkeleyomyces basicola causing black root rot on sweet potato in Mendoza, Argentina.
|
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2021 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/4898 |
| topic |
Ipomoea batatas podredumbre de raíz Chalara elegans enfermedad fúngica en batata Ipomoea batatas root rot Chalara elegans potato sweet potato fungal disease |
| topic_facet |
Ipomoea batatas podredumbre de raíz Chalara elegans enfermedad fúngica en batata Ipomoea batatas root rot Chalara elegans potato sweet potato fungal disease |
| work_keys_str_mv |
AT martinojuliaa firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT difeolilianadelvalle firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT pacciorettimauro firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT contardiclaraadriana firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT sanchezmiguela firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT ortegaleandroi firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina AT pastorsilvinae firstreportofberkeleyomycesbasicolasynonymousthielaviopsisbasicolaonrootsofsweetpotatoipomoeabatatasllaminargentina |
| _version_ |
1800220932345692160 |
| spelling |
I11-R107article-48982021-07-07T00:34:42Z First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina First report of Berkeleyomyces basicola (synonymous: Thielaviopsis basicola) on roots of sweet potato (Ipomoea batatas (L.) Lam) in Argentina Martino, Julia A. Di Feo, Liliana del Valle Paccioretti, Mauro Contardi, Clara Adriana Sanchez, Miguel A. Ortega, Leandro I. Pastor, Silvina E. Ipomoea batatas podredumbre de raíz Chalara elegans enfermedad fúngica en batata Ipomoea batatas root rot Chalara elegans potato sweet potato fungal disease Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields. Highlights: Sweet potato black root rot, new disease in Argentina. First report of Berkeleyomyces basicola causing black root rot on sweet potato in Mendoza, Argentina. Symptomatic sweet potato cv Arapey INIA samples were collected from a commercial production field in Colonia Molina, Guaymallén department, Mendoza province, Argentina. They showed dark rounded lesions, sometimes coalescing with white granular mycelium. Fungus was obtained from symptomatic sweet potatoes, which represented the generalized infection that affected the crop. They were seeded in PDA with streptomycin sulfate and incubated for seven days at 21°C, alternating white/black (UV400nm) light. Observations with an optical microscope revealed the presence of hyaline, not septated, cylindrical endoconidia with rounded ends. They were 8-16 μm length and 4–6 μm width. Phialides were 43-46 μm length, rounded bases (7-9 μm width) and tapering to the neck´s tip (4-6 μm width). Brown chlamydospores (aleuriospores), 9-13 μm length and 8-12 μm width, in chains of 2-8 spores were observed. For molecular identification, total genomic DNA was extracted. ITS fragment of 565 pb was amplified using ITS5/ITS4 primers and sequenced. The sequence indicated 99% identity with Berkeleyomyces basicola (synonymous: Thielaviopsis basicola). This was deposited in GenBank as (KX580957) (CBS: C430.74, Gen Bank accession number AF275482.1). This is the first report of B. basicola in sweet potato in Argentina, a potential threat to storage root yields. Highlights: Sweet potato black root rot, new disease in Argentina. First report of Berkeleyomyces basicola causing black root rot on sweet potato in Mendoza, Argentina. Facultad de Ciencias Agrarias-UNCuyo 2021-07-07 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/4898 10.48162/rev.39.027 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 53 No. 1 (2021): January-June; 283-287 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 53 Núm. 1 (2021): Enero-Junio; 283-287 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/4898/3554 https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
