Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina
Melissa officinalis L., so called "Melissa" or "Toronjil", is a perennial aromatic herb, whose leaves are used in traditional medicine as a carminative, digestive and sedative, both in simple as in mixtures. Meanwhile, Nepeta cataria L., commonly called "Cat mint" or &q...
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Facultad de Ciencias Agrarias-UNCuyo
2022
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I11-R107article-56012022-02-10T15:58:40Z Quantitative micrograph, HPLC and FTIR profiles of Melissa officinalis and Nepeta cataria (Lamiaceae) from Argentina Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina Petenatti, Marta E. Gette, María A. Camí, Gerardo E. Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR parámetros micrográficos cuantitativos Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR Quantitative micrographic parameters Melissa officinalis L., so called "Melissa" or "Toronjil", is a perennial aromatic herb, whose leaves are used in traditional medicine as a carminative, digestive and sedative, both in simple as in mixtures. Meanwhile, Nepeta cataria L., commonly called "Cat mint" or "Toronjil", with some similar properties, often replacing M. officinalis in the market, although their chemical composition is not completely matched, and contains an iridoid potentially toxic (nepetalactone). It is therefore necessary to establish diacritic parameters to differentiate these species, both at crude drug level, mixtures and extracts. Samples from various sources in Argentina were studied and documental specimens are preserved in the Herbarium UNSL. Anatomical sections were analyzed, and quantitative micrographic parameters were obtained, together with HPLC and FTIR spectra from methanolic and aqueous lyophilized extracts. Significant differences were detected in the prevailing smell foliage, morphology of leaves and inflorescences, trichomata type, palisade ratio, veinlet termination number, rosmarinic acid concentration (with distinctive HPLC profiles), and the CO/CH relationships obtained by FTIR from the extracts, that together allow adequate differentiation of both drugs, even when they were ground or powdered. Melissa officinalis L., llamada vulgarmente "melisa" o "toronjil", es una hierba perenne, aromática, cuyas hojas son empleadas en medicina popular como carminativo, digestivo y sedante, tanto en droga simple como en asociación. Por su parte Nepeta cataria L., llamada vulgarmente "menta de los gatos" y también "toronjil", con algunas propiedades similares, sustituye con frecuencia a M. officinalis en el mercado, aunque su composición química no es del todo coincidente y contiene un iridoide potencialmente tóxico (nepetalactona). Por ello es necesario establecer parámetros diacríticos para diferenciar ambas especies, a nivel de droga cruda, mezclas y extractos. Fueron estudiados especímenes de diversas procedencias en Argentina, y los ejemplares documentales son conservados en el Herbario UNSL. Se analizaron cortes anatómicos, se registraron los parámetros micrográficos cuantitativos y se obtuvieron espectros de HPLC y FTIR a partir de extractos metanólicos y acuosos liofilizados. Fueron detectadas diferencias significativas entre ambas especies en cuanto al aroma prevaleciente del follaje, la exomorfología de hojas e inflorescencias, los tipos tricomáticos, la relación de empalizada, el número de terminales de nerviación, la concentración de ácido rosmarínico (con perfiles HPLC distintivos), y las relaciones CO/CH obtenidas por FTIR a partir de los extractos, caracteres que en conjunto permiten una diferenciación adecuada de ambas drogas, aún cuando se presenten molidas o reducidas a polvo. Facultad de Ciencias Agrarias-UNCuyo 2022-02-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5601 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 46 No. 2 (2014): July-December; 15-27 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 46 Núm. 2 (2014): Julio-Diciembre; 15-27 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5601/4306 https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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R-107 |
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eng |
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Online |
author |
Petenatti, Marta E. Gette, María A. Camí, Gerardo E. |
spellingShingle |
Petenatti, Marta E. Gette, María A. Camí, Gerardo E. Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR parámetros micrográficos cuantitativos Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR Quantitative micrographic parameters |
author_facet |
Petenatti, Marta E. Gette, María A. Camí, Gerardo E. |
author_sort |
Petenatti, Marta E. |
title |
Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina |
title_short |
Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina |
title_full |
Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina |
title_fullStr |
Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina |
title_full_unstemmed |
Micrografía cuantitativa y perfiles de HPLC y FTIR de Melissa officinalis y Nepeta cataria (Lamiaceae) de Argentina |
title_sort |
quantitative micrograph, hplc and ftir profiles of melissa officinalis and nepeta cataria (lamiaceae) from argentina |
description |
Melissa officinalis L., so called "Melissa" or "Toronjil", is a perennial aromatic herb, whose leaves are used in traditional medicine as a carminative, digestive and sedative, both in simple as in mixtures. Meanwhile, Nepeta cataria L., commonly called "Cat mint" or "Toronjil", with some similar properties, often replacing M. officinalis in the market, although their chemical composition is not completely matched, and contains an iridoid potentially toxic (nepetalactone). It is therefore necessary to establish diacritic parameters to differentiate these species, both at crude drug level, mixtures and extracts. Samples from various sources in Argentina were studied and documental specimens are preserved in the Herbarium UNSL. Anatomical sections were analyzed, and quantitative micrographic parameters were obtained, together with HPLC and FTIR spectra from methanolic and aqueous lyophilized extracts. Significant differences were detected in the prevailing smell foliage, morphology of leaves and inflorescences, trichomata type, palisade ratio, veinlet termination number, rosmarinic acid concentration (with distinctive HPLC profiles), and the CO/CH relationships obtained by FTIR from the extracts, that together allow adequate differentiation of both drugs, even when they were ground or powdered. |
publisher |
Facultad de Ciencias Agrarias-UNCuyo |
publishDate |
2022 |
url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5601 |
topic |
Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR parámetros micrográficos cuantitativos Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR Quantitative micrographic parameters |
topic_facet |
Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR parámetros micrográficos cuantitativos Melissa officinalis Melissae folium Nepeta cataria HPLC FTIR Quantitative micrographic parameters |
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