Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea
The aims of this work were: to determine feasibility of the combination of two biological control methods: application of antagonistic fungus Trichoderma spp. and biofumigation with the aerial part of Brassica juncea in the end of fruiting stage; to evaluate their effect on the growth of the pathoge...
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| Formato: | Online |
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Facultad de Ciencias Agrarias-UNCuyo
2022
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5603 |
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I11-R107article-5603 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
| language |
spa |
| format |
Online |
| author |
Perniola, Omar Salvador Staltari, Sebastián Chorzempa, Silvia Elena Astiz Gassó, Marta Mónica Molina, María del Carmen |
| spellingShingle |
Perniola, Omar Salvador Staltari, Sebastián Chorzempa, Silvia Elena Astiz Gassó, Marta Mónica Molina, María del Carmen Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea in vitro dual culture antagonism biocontrol biofumigation Brassicaceae in vitro cultivo dual antagonismo biocontrol biofumigación Brassicáceas |
| author_facet |
Perniola, Omar Salvador Staltari, Sebastián Chorzempa, Silvia Elena Astiz Gassó, Marta Mónica Molina, María del Carmen |
| author_sort |
Perniola, Omar Salvador |
| title |
Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea |
| title_short |
Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea |
| title_full |
Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea |
| title_fullStr |
Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea |
| title_full_unstemmed |
Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea |
| title_sort |
biological control of fusarium graminearum: use of trichoderma spp. and biofumigation with aerial part of brassica juncea |
| description |
The aims of this work were: to determine feasibility of the combination of two biological control methods: application of antagonistic fungus Trichoderma spp. and biofumigation with the aerial part of Brassica juncea in the end of fruiting stage; to evaluate their effect on the growth of the pathogen Fusarium graminearum. Two doses (5 and 10 g) of triturated plant material from B. juncea were placed in plastic recipients. Petri dishes with potato glucose agar medium 2% and a disc inoculated with F. graminearum, or Trichoderma spp. or both fungi, were placed on top of the plant material. Plastic recipients were then closed and incubated at 25±2°C in darkness for 7 days. After that, the diameter of the colonies was measured. The results indicated that: i) when Trichoderma spp. and F. graminearum were biofumigated separately, fungistatic effect was not observed, ii) without biofumigant, Trichoderma spp. significantly inhibited growth of F. graminearum colonies, iii) the combination of Trichoderma spp. and the biofumigation with B. juncea showed synergic effect on growth control of F. graminearum. These in vitro results suggest that the growth of Trichoderma spp. and its potential biocontrol effect of F. graminearum, are not affected by biofumigation with B. juncea. Also, the combination of Trichoderma spp. and biofumigation with B. juncea, would have synergic effect on growth control of F. graminearum. |
| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2022 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5603 |
| topic |
in vitro dual culture antagonism biocontrol biofumigation Brassicaceae in vitro cultivo dual antagonismo biocontrol biofumigación Brassicáceas |
| topic_facet |
in vitro dual culture antagonism biocontrol biofumigation Brassicaceae in vitro cultivo dual antagonismo biocontrol biofumigación Brassicáceas |
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1800220935365591040 |
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I11-R107article-56032022-02-10T15:57:47Z Biological control of Fusarium graminearum: use of Trichoderma spp. and biofumigation with aerial part of Brassica juncea Control biológico de Fusarium graminearum: utilización de Trichoderma spp. y biofumigación con parte aérea de Brassica juncea Perniola, Omar Salvador Staltari, Sebastián Chorzempa, Silvia Elena Astiz Gassó, Marta Mónica Molina, María del Carmen in vitro dual culture antagonism biocontrol biofumigation Brassicaceae in vitro cultivo dual antagonismo biocontrol biofumigación Brassicáceas The aims of this work were: to determine feasibility of the combination of two biological control methods: application of antagonistic fungus Trichoderma spp. and biofumigation with the aerial part of Brassica juncea in the end of fruiting stage; to evaluate their effect on the growth of the pathogen Fusarium graminearum. Two doses (5 and 10 g) of triturated plant material from B. juncea were placed in plastic recipients. Petri dishes with potato glucose agar medium 2% and a disc inoculated with F. graminearum, or Trichoderma spp. or both fungi, were placed on top of the plant material. Plastic recipients were then closed and incubated at 25±2°C in darkness for 7 days. After that, the diameter of the colonies was measured. The results indicated that: i) when Trichoderma spp. and F. graminearum were biofumigated separately, fungistatic effect was not observed, ii) without biofumigant, Trichoderma spp. significantly inhibited growth of F. graminearum colonies, iii) the combination of Trichoderma spp. and the biofumigation with B. juncea showed synergic effect on growth control of F. graminearum. These in vitro results suggest that the growth of Trichoderma spp. and its potential biocontrol effect of F. graminearum, are not affected by biofumigation with B. juncea. Also, the combination of Trichoderma spp. and biofumigation with B. juncea, would have synergic effect on growth control of F. graminearum. Los objetivos de este trabajo fueron: determinar la factibilidad de la utilización combinada de dos métodos de control biológico: la aplicación del hongo antagonista Trichoderma spp. y la biofumigación con la parte aérea de Brassica juncea en el estadio de fin de fructificación; evaluar su efecto sobre el crecimiento del patógeno Fusarium graminearum. Se trituraron plantas de B. juncea y se colocaron en recipientes de plástico en dosis de 5 y 10 g. Sobre el material triturado se apoyó una caja de Petri con agar papa glucosado al 2%, que contenía un disco con micelio de F. graminearum o Trichoderma spp. o ambos hongos. Los recipientes de plástico se cerraron e incubaron a 25±2°C en oscuridad durante 7 días. Finalizado este período, se midió el diámetro de las colonias. Se obtuvieron los siguientes resultados: i) cuando se biofumigaron por separado, no se observó efecto fungistático de B. juncea sobre Trichoderma spp. ni sobre F. graminearum; ii) en ausencia del biofumigante, Trichoderma spp. inhibió significativamente el crecimiento de las colonias de F. graminearum, iii) la combinación de Trichoderma spp. y la biofumigación con B. juncea mostró un efecto sinérgico sobre el control del crecimiento miceliar de F. graminearum. Los resultados in vitro sugieren que el crecimiento de Trichoderma spp. y su potencial efecto de biocontrol sobre F. graminearum, no son afectados por la biofumigación con B. juncea. La utilización combinada de Trichoderma spp. y la biofumigación con B. juncea, tendría un efecto sinérgico sobre el control del crecimiento de F. graminearum. Facultad de Ciencias Agrarias-UNCuyo 2022-02-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5603 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 46 No. 2 (2014): July-December; 45-56 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 46 Núm. 2 (2014): Julio-Diciembre; 45-56 1853-8665 0370-4661 spa https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/5603/4308 https://creativecommons.org/licenses/by-nc-sa/3.0/deed.es |
