Bacterias lácticas nativas de interés industrial aisladas a partir de arándanos frescos (Vaccinium corymbosum)

Lactic acid bacteria may have functional properties that can be used for the production of innovative fruit foods. The objective of this work was to isolate native strains of lactic acid bacteria with properties of industrial interest from fresh blueberries.  Native strains were isolated on...

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Detalles Bibliográficos
Autores principales: Ortiz, Silvia Graciela, Tunez, Juan Ignacio, Gallo, Alicia, Raffellini, Silvia
Formato: Online
Lenguaje:spa
Publicado: Facultad de Ciencias Aplicadas a la Industria 2022
Acceso en línea:https://revistas.uncu.edu.ar/ojs3/index.php/revicap/article/view/5984
Descripción
Sumario:Lactic acid bacteria may have functional properties that can be used for the production of innovative fruit foods. The objective of this work was to isolate native strains of lactic acid bacteria with properties of industrial interest from fresh blueberries.  Native strains were isolated on MRS agar and subjected to preliminary identification. Antagonistic activity against pathogenic and indicator microorganisms was determined. Other tests carried out were: resistance to pH 3; growth at different temperatures, bile salts (1%) and high concentration of sodium chloride (6.5%); production of exopolysaccharides (EPS); and characterization of extracellular antimicrobial metabolites. Ten native strains preliminarily characterized as lactic acid bacteria were isolated.  The Ar2 strain, identified by molecular methods as Weissella confusa, showed the highest inhibitory activity against Escherichia coli, Listeria monocytogenes and Salmonella Enteritidis. In addition, it resisted pH 3, grew in MRS medium added with bile salts (1%), produced EPS and extracellular bacteriocin-like metabolites with anti-Listeria monocytogenes effect. Consequently, the native lactic strain Weissella confusa Ar2 could potentially be applied in the production of innovative fruit foods either as a bioprotective microorganism, as a producer of natural thickeners or as a probiotic culture.   Keywords: Lactic acid bacteria, fruit, bioprotection, probiotics, exopolysaccharides