Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina
Squash landraces (Cucurbita maxima) are maintained by small farmers as a major nutritional food. Twenty seven of these landraces were collected in Argentinian Andean Valleys and morphologically characterized. Genetic diversity was evaluated with microsatellite markers designed for Cucurbita pepo an...
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| Formato: | Online |
| Lenguaje: | eng |
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Facultad de Ciencias Agrarias-UNCuyo
2020
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| Acceso en línea: | https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3033 |
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I11-R107article-3033 |
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Universidad Nacional de Cuyo |
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Revistas en línea |
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Revistas en línea |
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Revista de la Facultad de Ciencias Agrarias |
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Revista de la Facultad de Ciencias Agrarias |
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I-11 |
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R-107 |
| language |
eng |
| format |
Online |
| author |
Lorello, Inés María García Lampasona, Sandra Claudia Peralta, Iris Edith |
| spellingShingle |
Lorello, Inés María García Lampasona, Sandra Claudia Peralta, Iris Edith Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina Marcadores microsatélites diversidad genética bancos de germoplasma recursos genéticos zapallo Cucurbita maxima Microsatellite markers genetic diversity germplasm banks genetic resources squash landraces Cucurbita maxima |
| author_facet |
Lorello, Inés María García Lampasona, Sandra Claudia Peralta, Iris Edith |
| author_sort |
Lorello, Inés María |
| title |
Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina |
| title_short |
Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina |
| title_full |
Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina |
| title_fullStr |
Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina |
| title_full_unstemmed |
Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina |
| title_sort |
genetic diversity of squash landraces (cucurbita maxima) collected in andean valleys of argentina |
| description |
Squash landraces (Cucurbita maxima) are maintained by small farmers as a major nutritional food. Twenty seven of these landraces were collected in Argentinian Andean Valleys and morphologically characterized. Genetic diversity was evaluated with microsatellite markers designed for Cucurbita pepo and Cucumis melo and evaluated for the first time in C. maxima. Seven microsatellite primers detected 26 alleles with 3.10 average alleles per locus. The Genetic Diversity reached an average of 0.26; a Polymorphic Information Content (PIC) of 0.20 and 45.5% of polymorphic loci. Higher diversity was found at intra population level. No evidence of lineal correlation between the observed diversity and the geographical distribution of squash landraces was found. Results demonstrate a moderate genetic diversity for all populations, with a wide range of variation in different groups. A subgroup of 10 populations with the highest levels of genetic diversity was considered for maintenance within core collections in the Vegetable Crop Germplasm Bank of Agricultural Research Station (EEA) La Consulta, Mendoza, National Institute of Agricultural Technology (INTA). Anthropogenic and environmental processes, mainly abandonment of cultivated areas and frequent droughts could erode squash landraces diversity. Conservational strategies and new collecting expeditions can be decided based on the genetic diversity found.
Highlights
Argentinean squashe's landraces from different Andean environments were characterized for the first time by molecular markers.
A technique was developed to generate the amplification products, and to detect useful markers for the conservation of C. maxima genetic resources in germplasm banks.
Diversification strategies of food production and seed exchange are deeply rooted in Andean culture, which contributes to the intrapopulation diversity observed.
Catamarca and Jujuy were the Provinces with the greatest genetic diversity for cultivated squashes.
The great morphological diversity observed would be the result of species adaptation to Andean heterogeneous ecological environments, and anthropic selection.
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| publisher |
Facultad de Ciencias Agrarias-UNCuyo |
| publishDate |
2020 |
| url |
https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3033 |
| topic |
Marcadores microsatélites diversidad genética bancos de germoplasma recursos genéticos zapallo Cucurbita maxima Microsatellite markers genetic diversity germplasm banks genetic resources squash landraces Cucurbita maxima |
| topic_facet |
Marcadores microsatélites diversidad genética bancos de germoplasma recursos genéticos zapallo Cucurbita maxima Microsatellite markers genetic diversity germplasm banks genetic resources squash landraces Cucurbita maxima |
| work_keys_str_mv |
AT lorelloinesmaria geneticdiversityofsquashlandracescucurbitamaximacollectedinandeanvalleysofargentina AT garcialampasonasandraclaudia geneticdiversityofsquashlandracescucurbitamaximacollectedinandeanvalleysofargentina AT peraltairisedith geneticdiversityofsquashlandracescucurbitamaximacollectedinandeanvalleysofargentina |
| _version_ |
1800220895100272640 |
| spelling |
I11-R107article-30332020-07-06T15:05:37Z Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina Genetic diversity of squash landraces (Cucurbita maxima) collected in Andean Valleys of Argentina Lorello, Inés María García Lampasona, Sandra Claudia Peralta, Iris Edith Marcadores microsatélites diversidad genética bancos de germoplasma recursos genéticos zapallo Cucurbita maxima Microsatellite markers genetic diversity germplasm banks genetic resources squash landraces Cucurbita maxima Squash landraces (Cucurbita maxima) are maintained by small farmers as a major nutritional food. Twenty seven of these landraces were collected in Argentinian Andean Valleys and morphologically characterized. Genetic diversity was evaluated with microsatellite markers designed for Cucurbita pepo and Cucumis melo and evaluated for the first time in C. maxima. Seven microsatellite primers detected 26 alleles with 3.10 average alleles per locus. The Genetic Diversity reached an average of 0.26; a Polymorphic Information Content (PIC) of 0.20 and 45.5% of polymorphic loci. Higher diversity was found at intra population level. No evidence of lineal correlation between the observed diversity and the geographical distribution of squash landraces was found. Results demonstrate a moderate genetic diversity for all populations, with a wide range of variation in different groups. A subgroup of 10 populations with the highest levels of genetic diversity was considered for maintenance within core collections in the Vegetable Crop Germplasm Bank of Agricultural Research Station (EEA) La Consulta, Mendoza, National Institute of Agricultural Technology (INTA). Anthropogenic and environmental processes, mainly abandonment of cultivated areas and frequent droughts could erode squash landraces diversity. Conservational strategies and new collecting expeditions can be decided based on the genetic diversity found. Highlights Argentinean squashe's landraces from different Andean environments were characterized for the first time by molecular markers. A technique was developed to generate the amplification products, and to detect useful markers for the conservation of C. maxima genetic resources in germplasm banks. Diversification strategies of food production and seed exchange are deeply rooted in Andean culture, which contributes to the intrapopulation diversity observed. Catamarca and Jujuy were the Provinces with the greatest genetic diversity for cultivated squashes. The great morphological diversity observed would be the result of species adaptation to Andean heterogeneous ecological environments, and anthropic selection. Squash landraces (Cucurbita maxima) are maintained by small farmers as a major nutritional food. Twenty seven of these landraces were collected in Argentinian Andean Valleys and morphologically characterized. Genetic diversity was evaluated with microsatellite markers designed for Cucurbita pepo and Cucumis melo and evaluated for the first time in C. maxima. Seven microsatellite primers detected 26 alleles with 3.10 average alleles per locus. The Genetic Diversity reached an average of 0.26; a Polymorphic Information Content (PIC) of 0.20 and 45.5% of polymorphic loci. Higher diversity was found at intra population level. No evidence of lineal correlation between the observed diversity and the geographical distribution of squash landraces was found. Results demonstrate a moderate genetic diversity for all populations, with a wide range of variation in different groups. A subgroup of 10 populations with the highest levels of genetic diversity was considered for maintenance within core collections in the Vegetable Crop Germplasm Bank of Agricultural Research Station (EEA) La Consulta, Mendoza, National Institute of Agricultural Technology (INTA). Anthropogenic and environmental processes, mainly abandonment of cultivated areas and frequent droughts could erode squash landraces diversity. Conservational strategies and new collecting expeditions can be decided based on the genetic diversity found. Highlights Argentinean squashe's landraces from different Andean environments were characterized for the first time by molecular markers. A technique was developed to generate the amplification products, and to detect useful markers for the conservation of C. maxima genetic resources in germplasm banks. Diversification strategies of food production and seed exchange are deeply rooted in Andean culture, which contributes to the intrapopulation diversity observed. Catamarca and Jujuy were the Provinces with the greatest genetic diversity for cultivated squashes. The great morphological diversity observed would be the result of species adaptation to Andean heterogeneous ecological environments, and anthropic selection. Facultad de Ciencias Agrarias-UNCuyo 2020-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3033 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 No. 1 (2020): January-June; 293-313 Revista de la Facultad de Ciencias Agrarias UNCuyo; Vol. 52 Núm. 1 (2020): Enero-Junio; 293-313 1853-8665 0370-4661 eng https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3033/2204 https://revistas.uncu.edu.ar/ojs3/index.php/RFCA/article/view/3033/2626 |
